| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL3.1[Nluc/minP] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3151 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
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pNL3.1[Nluc/minP]载体质粒基本信息
pNL3.1[Nluc/minP]质粒图谱载体图谱和pNL3.1[Nluc/minP]载体序列质粒序列多克隆位点信息
![pNL3.1[Nluc-minP]载体图谱](/uploads/载体图谱/pNL3.1[Nluc-minP]载体图谱.png)
pNL3.1[Nluc/minP]质粒载体简介
pNL3.1[Nluc/minP]质粒序列载体序列
LOCUS pNL3.1[Nluc minP] 3151 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Vector with a minimal promoter for measuring the activity of
transcriptional response elements using a NanoLuc(R) luciferase
assay.
ACCESSION JQ437373
VERSION .
KEYWORDS pNL3.1[Nluc minP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3151)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Wednesday, February 26, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3151
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
promoter 77..108
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 141..656
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
polyA_signal 705..826
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1245..1833)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2033..2893)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 2998..3046
/note="synthetic polyadenylation signal"
misc_feature 3060..3151
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
121 gtactgttgg taaagccacc atggtcttca cactcgaaga tttcgttggg gactggcgac
181 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
241 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
301 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
361 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
421 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
481 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
541 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
601 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaaggcc
661 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
721 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
781 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
841 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
901 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
961 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
1021 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
1081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
1141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
1201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
1261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
1321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
1381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
1441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
1501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
1561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
1621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
1681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
1741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
1801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
1861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
1921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
1981 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
2041 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
2101 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
2161 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
2221 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
2281 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
2341 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
2401 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
2461 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
2521 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
2581 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
2641 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
2701 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
2761 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
2821 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
2881 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
2941 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
3001 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
3061 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
3121 agtgcaagtg caggtgccag aacatttctc t
//
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